Pha-pro Integrations ((better)) -

| Component | Prokaryotic Host | Integration Strategy | |-----------|----------------|----------------------| | | Cupriavidus necator (PHB), Pseudomonas spp. (mcl-PHA) | Fuse PhaC with phasin (PhaP) for granule binding; use dual-plasmid systems | | Carbon flux | TCA cycle ↔ fatty acid β-oxidation | Knockout fadB (β-oxidation) + overexpress fadD (acyl-CoA ligase) | | Precursor supply | Acetyl-CoA → 3HB (PHB) or (R)-3HA (mcl-PHA) | Integrate phaA , phaB operon under inducible promoter (e.g., P₁₅₅₃, Pₗₐc) |