[Poly(A)+ RNA Template] │ ▼ (RT Primer & Maxima H- Reverse Transcriptase) [First-Strand Synthesis & UMI Incorporation] │ ▼ (Strand-Switching Oligo) [Full-Length cDNA Template Generation] │ ▼ (LongAmp Taq PCR Amplification with 5' Tagged Barcode Primers) [Amplified Barcoded ds-cDNA Pool] │ ▼ (Ligase-Free Rapid Adapter Attachment via 5' Tags) [Final Sequence-Ready Library] 1. Reverse Transcription and Strand Switching
The generated full-length cDNA undergoes PCR amplification using . The kit's barcode primers feature specialized 5' tags. These primers amplify the cDNA while attaching one of the 24 distinct barcodes to each respective sample. 3. Ligase-Free Adapter Ligation sqk-pcb114.24
Supports up to 24 samples simultaneously using unique barcodes. [Poly(A)+ RNA Template] │ ▼ (RT Primer &
This content is provided for informational purposes. Always refer to the official "Ligation Sequencing Kit (SQK-PCB114)" Protocol document provided by Oxford Nanopore Technologies for precise handling instructions and safety warnings. These primers amplify the cDNA while attaching one
Ligation Sequencing Kit Catalog Number: SQK-PCB114.24 Manufacturer: Oxford Nanopore Technologies Application: Genomic DNA & RNA Sequencing