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ГлавнаяBuffer ATL often contains or detergents that can be irritants.
| Issue | Possible Cause | Solution related to Buffer ATL | | :--- | :--- | :--- | | | Incomplete Lysis | Increase incubation time with Buffer ATL + Proteinase K. Ensure tissue was cut small enough. | | Clogged Column | Too much debris | Centrifuge the lysate after Buffer ATL digestion to pellet debris, then transfer supernatant to the column. | | Degraded DNA | Nuclease activity | Ensure Buffer ATL volume is correct to create the proper osmotic pressure; ensure Proteinase K was added immediately. |
