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From May 2022 to April 2024, a total of 1 200 fish specimens representing 15 teleost species were collected from 12 sites along the Yangtze River and its tributaries (Table 1). Simultaneously, 300 freshwater gastropods ( Radix auricularia and Pomacea canaliculata ) were sampled to assess potential intermediate hosts. All specimens were transported on ice to the laboratory within 12 h of capture. lia lin parasited